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Recombinant Protein - RPS6KA6 (RSK4) (1 - 745)

Name: RPS6KA6 (RSK4) (1 - 745)
Price per aliquot (100µg): £110.00
Long Name:
ribosomal protein S6 kinase A6
Synonyms:
ribosomal protein S6 kinase, 90kD, polypeptide 6, PP90RSK4, RSK-4
Catalog Number (DU Number):
DU63293
Accession:
NM_014496.5
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
Long S6 peptide (KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK)
Calculated molecular mass:
Mono-Isotopic Mass: 110, 625.15 daltons
Average Mass: 110, 696.21 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.88
Native Sequence:
Amino acids M1 – L745 (end) of human RSK4. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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