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Recombinant Protein - CaMK1B (1 - 426)

Name: CaMK1B (1 - 426)
Price per aliquot (100µg): £110.00
Long Name:
calcium/calmodulin dependent protein kinase IB
Synonyms:
CAMK1B, calcium/calmodulin-dependent protein kinase IB, PNCK, pregnancy up-regulated nonubiquitous CaM kinase, pregnancy upregulated non-ubiquitously expressed CaM kinase, pregnancy up-regulated non-ubiquitously expressed CaM kinase, MGC45419, CaMK1b
Catalog Number (DU Number):
DU61079
Accession:
NM_001039582.3
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc, 0.1 mM CaCl2, 1 μM Calmodulin
Enzymatic Substrate:
KKALRRQETVDAL; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 74, 107.74 daltons
Average Mass: 74, 154.91 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.29
Native Sequence:
Amino acids M1 – W426 (end) of human CaMK1B. Residue M235 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
EcoR1 - Not1 sites of pGEX6P-1

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