Recombinant Protein - CaMK1G (1 - 476)

Name: CaMK1G (1 - 476)
Price per aliquot (100µg): £110.00
Long Name:
calcium/calmodulin dependent protein kinase IG
Synonyms:
CAMK1G, calcium/calmodulin-dependent protein kinase IG, VWS1, CLICKIII, dJ272L16.1, CLICK3
Catalog Number (DU Number):
DU30189
Accession:
NM_020439.3
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc, 0.1 mM CaCl2, 1 μM Calmodulin
Enzymatic Substrate:
YLRRRLSDSNF; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 79, 859.39 daltons
Average Mass: 79, 911.10 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
6.75
Native Sequence:
Amino acids M1 – M476 (end) of human CaMK1G. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
Bgl2 - Not1 into BamH1- Not1 site of pGEX6P-1

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