Recombinant Protein - EPH A3 (568 - 983)

Name: EPH A3 (568 - 983)
Price per aliquot (100µg): £110.00
Long Name:
EPH receptor A3
Synonyms:
EPHA3, ETK, ETK1, TYRO4, HEK, HEK4, EphA3
Catalog Number (DU Number):
DU63469
Accession:
NM_005233.6
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu:Tyr (4:1) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 73, 236.39 daltons
Average Mass: 73, 283.43 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical PI:
6.75
Native Sequence:
Amino acids C568 – V983 (end) of human EPH A3. Residue 232C of the fusion protein is equivalent to C568 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a L856Q mutation. Residues L856 is equivalent to Q520 of the fusion protein
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BamH1 and Not1 site of pGEX 6P-1

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