Recombinant Protein - ASK1 (2 - 1374)

Name: ASK1 (2 - 1374)
Price per aliquot (100µg): £110.00
Long Name:
mitogen-activated protein kinase kinase kinase 5
Synonyms:
MEKK5, MAPKKK5
Catalog Number (DU Number):
DU4707
Accession:
NM_005923
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Myelin Basic Protein; Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 181, 792.47 daltons
Average Mass: 181, 907.61 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.57
Native Sequence:
Amino acids S2 – T1374 (end) of human ASK1. Residue S238 of the fusion protein is equivalent to S2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
Sal1 sites of pGEX 6P-3

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