Recombinant Protein - EPH A1 (568 - 976)

Name: EPH A1 (568 - 976)
Price per aliquot (100µg): £110.00
Long Name:
EPH receptor A1
Synonyms:
EPHA1, EPHT, EPHT1, EPH
Catalog Number (DU Number):
DU63274
Accession:
NM_005232.5
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
EFPIYDFLPAKKK Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 74, 059.48 daltons
Average Mass: 74, 107.19 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical PI:
5.96
Native Sequence:
Amino acids F568 – D976 (end) of human EPH A1. Residue F235 of the fusion protein is equivalent to F568 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
EcoR1 and Not1 site of pGEX 6P-1

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