Recombinant Protein - MST3 (19 - 289)

Name: MST3 (19 - 289)
Price per aliquot (100µg): £110.00
Long Name:
serine/threonine kinase 24
Synonyms:
STK24, MST-3, MST3B, STE20, STE20-like kinase 3, sterile 20-like kinase 3
Catalog Number (DU Number):
DU30889
Accession:
Q9Y6E0-2
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Cobalt Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Myelin Basic Protein (MBP)
Calculated molecular mass:
Mono-Isotopic Mass: 33, 199.24 daltons
Average Mass: 33, 220.06 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.67
Native Sequence:
Amino acids D19 – D289 (end) of human CSNK1G3. Residue D26 of the fusion protein is equivalent to D19 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
PreScission (LEVLFQGP) residues 14 - 21
Cloning Sites:
BamH1 and Not1 sites of pET15b

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