Recombinant Protein - EGFR (695 - 1210)

Name: EGFR (695 - 1210)
Price per aliquot (100µg): £110.00
Long Name:
Epidermal Growth Factor Receptor
Synonyms:
ERBB1, erb-b2 receptor tyrosine kinase 1, HER1, ERBB
Catalog Number (DU Number):
DU 63647
Accession:
NM_005228.4
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Enzymatic Assay Format:
32P filter binding
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 10 mM DTT, 2 mM MnCl2
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 0.1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 84, 555.56 daltons
Average Mass: 84, 609.73 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
5.46
Native Sequence:
Amino acids G695 – A1210 (end) of human EGFR. Residue S231of the fusion protein is equivalent to G695 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission site (LEVLFQGP) residues 221 – 228
Cloning Sites:
Xho1 and HindIII sites into Sal1 and HindIII followed by frame correction of pFastBac GST 6P1

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