Recombinant Protein - PYK2 (2 - 1009)

Name: PYK2 (2 - 1009)
Price per aliquot (100µg): £110.00
Long Name:
protein tyrosine kinase 2 beta
Synonyms:
PTK2B, FAK2, CADTK, CAKB, PTK, RAFTK
Catalog Number (DU Number):
1019
Accession:
NM_173176.2
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni-NTA agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 5 mM MnCl2
Enzymatic Substrate:
Poly Glu Tyr (4:1)
Calculated molecular mass:
Mono-Isotopic Mass: 119, 382.69 daltons
Average Mass: 119, 459.41 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.84
Native Sequence:
Amino acids S2 – E1009 (end) of human PYK2. Residue S31 of the fusion protein is equivalent to S2 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQ) residues 18 - 24
Cloning Sites:
EcoR1 and Xba1 sites of pFastBac.Hta

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