Recombinant Protein - CK1 gamma 3 (1 - 423)

Name: CK1 gamma 3 (1 - 423)
Price per aliquot (100µg): £110.00
Long Name:
Casein Kinase 1 Gamma 3
Synonyms:
CSNK1G3, casein kinase 1, gamma 3, CK1gamma3
Catalog Number (DU Number):
DU35305
Accession:
AAH47567.1
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
N-terminal GST
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
KRRRALS*VASLPGL (where S* is phospho Ser) Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 75, 674.27 daltons
Average Mass: 75, 723.09 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
8.61
Native Sequence:
Amino acids M1 – K423 (end) of human CSNK1G3. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and Not1 sites of pGEX 6P-1

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