Recombinant Protein - Calmodulin 2 (1 - 149)

Name: Calmodulin 2 (1 - 149)
Price per aliquot (100µg): £100.00
Synonyms:
CALM2, PHKD, phosphorylase kinase delta
Catalog Number (DU Number):
DU11423
Accession:
NM_001743
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 43, 633.47 daltons
Average Mass: 43, 661.75 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical PI:
4.64
Native Sequence:
Amino acids M1 – K149 (end) of human Calmodulin 2. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-3

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