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COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Recombinant Protein - Calmodulin 2 (1 - 149)

Name: Calmodulin 2 (1 - 149)
Price per aliquot (100µg): £110.00
Synonyms:
CALM2, PHKD, phosphorylase kinase delta
Catalog Number (DU Number):
DU11423
Accession:
NM_001743
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 43, 633.47 daltons
Average Mass: 43, 661.75 daltons
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical PI:
4.64
Native Sequence:
Amino acids M1 – K149 (end) of human Calmodulin 2. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-3

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