Recombinant Protein - RET (658 - 1114)

Name: RET (658 - 1114)
Price per aliquot (100µg): £110.00
Long Name:
RET proto-oncogene, receptor tyrosine kinase
Synonyms:
Hirschsprung disease 1, HSCR1, MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, cadherin-related family member 16, CDHF12, CDHR16, PTC, rearranged during transfection, RET receptor tyrosine kinase, RET51
Catalog Number (DU Number):
DU58691
Accession:
P07949-1
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
EAIYAAPFAKKK Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 78, 974.81 daltons
Average Mass: 79. 025.60 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
6.20
Native Sequence:
Amino acids H658 – S1114 (end) of human RET. Residue H238 of the fusion protein is equivalent to H658 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission site (LEVLFQGP) residues 221 – 228
Cloning Sites:
Sal1 and Not1 sites of pFastBac GST 6P3

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