Recombinant Protein - DGK gamma (1 -752)

Name: DGK gamma (1 -752)
Price per aliquot (100µg): £100.00
Long Name:
Diacylglycerol Kinase Gamma
Synonyms:
DGKG, DAGK3, DGK-gamma, DGK gamma
Catalog Number (DU Number):
DU63057
Accession:
BC112363
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GST Agarose
Enzymatic Assay Format:
ADP Glo
Enzymatic Buffer:
20 mM Tris-HCl, 67 mM KCl, 1 mM DTT, 0.05 mg/ml BSA, 10 mM MgCl
Enzymatic Substrate:
Diacylglycerol Final concentration: 0.1 mM
Calculated molecular mass:
Mono-Isotopic Mass: 112, 078.89 daltons
Average Mass: 112, 151.66 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM HEPES pH 7.0, 150 mM NaCl, 20 % glycerol, 5 mM DTT
Storage Temperature:
-70 °C
Theoretical pI:
5.97
Native Sequence:
Amino acids M1 – D752 (end) of human DGK gamma. Residue M239 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
Sal1 + 2/HindIII sites into pFastBacDual GST

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