Recombinant Protein - MRCK beta (1 - 473)

Name: MRCK beta (1 - 473)
Price per aliquot (100µg): £110.00
Long Name:
CDC42 binding protein kinase beta
Synonyms:
CDC42BPB, KIAA1124, MRCKB
Catalog Number (DU Number):
DU27777
Accession:
NM_006035.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 81, 377.68 daltons
Average Mass: 81, 430.17 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical pI:
5.16
Native Sequence:
Amino acids M1 – G473 (end residue is T1711) of human MRCK beta. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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