Recombinant Protein - p53 (1 - 393)

Name: p53 (1 - 393)
Price per aliquot (100µg): £110.00
Long Name:
tumor protein p53
Synonyms:
TP53, LFS1, Li-Fraumeni syndrome
Catalog Number (DU Number):
DU 51465
Accession:
AAG28785.1
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 70, 491.06 daltons
Average Mass: 70, 536.41 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
6.18
Native Sequence:
Amino acids M1 – D393 (end) of human p53. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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