Recombinant Protein - CLK3 (1 - 490)

Name: CLK3 (1 - 490)
Price per aliquot (100µg): £110.00
Long Name:
CDC like kinase 3
Synonyms:
CDC-like kinase 3
Catalog Number (DU Number):
58727
Accession:
NM_003992.4
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Seoharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
RNRYRDVSPFDHSR Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 85, 358.01 daltons
Average Mass: 85, 412.56 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
9.12
Native Sequence:
Amino acids M1 – K490 (end residue) of human CLK3. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1sites of pFastBac Dual.

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