Recombinant Protein - NEK1 (1 - 1286)

Name: NEK1 (1 - 1286)
Price per aliquot (100µg): £110.00
Long Name:
NIMA related kinase 1
Synonyms:
KIAA1901, NY-REN-55
Catalog Number (DU Number):
DU 41383
Accession:
NM_001199397.1
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Enzymatic Substrate:
Myelin Basic Protein (MBP)
Calculated molecular mass:
Mono-Isotopic Mass: 172, 530.46 daltons
Average Mass: 172, 637.89 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical PI:
5.72
Native Sequence:
Amino acids M1 – E1286 (end residue) of human NEK1. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1sites of pFastBac Dual.

Do you need any help? Please get in touch and we’ll be happy to lend a hand.