Recombinant Protein - PLK3 (1 - 646)

Name: PLK3 (1 - 646)
Price per aliquot (100µg): £110.00
Long Name:
Polo Like Kinase 3
Synonyms:
CNK, cytokine-inducible kinase, polo-like kinase 3, FNK, PRK, PLK-3
Catalog Number (DU Number):
DU 35183
Accession:
NM_004073.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Enzymatic Substrate:
ISDELMDATFADQEAKKK
Calculated molecular mass:
Mono-Isotopic Mass: 98, 390.86 daltons
Average Mass: 98, 453.49 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
8.76
Native Sequence:
Amino acids M1 – A646 (end) of human PLK3. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 sites of pGEX6P-1

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