Recombinant Protein - RAB32 (1 - 225)

Name: RAB32 (1 - 225)
Price per aliquot (100µg): £110.00
Long Name:
Rab32 member RAS oncogene family (RAB32)
Catalog Number (DU Number):
DU58222
Accession:
NM_006834.4
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Cleaved
Purification Method:
GSH-Sepharose, Cleavage of GST-SUMO and Gel filtration
Calculated molecular mass:
Mono-Isotopic Mass: 24,981.69 daltons [After tag cleavage]
Average Mass: 24, 997.29 daltons [After tag cleavage]
Purity:
>80 %
Storage Buffer:
50 mM HEPES pH 8, 150 mM NaCL,10 µM GDP, 0. 5m M TCEP
Storage Temperature:
-70 °C
Theoretical PI:
6.08 [After tag cleavage]
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – C225 (end) of human RAB32. Residue M325 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. SUMO is located at residues 229 - 324.
Protease Cleavage:
SENP1 cleavage of SUMO
Cloning Sites:
BamH1 and Xho1 sites of pGex6P-1-SUMO

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