COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Recombinant Protein - RAB32 (1 - 225)

Name: RAB32 (1 - 225)
Price per aliquot (100µg): £110.00
Long Name:
Rab32 member RAS oncogene family (RAB32)
Catalog Number (DU Number):
DU58222
Accession:
NM_006834.4
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Cleaved
Purification Method:
GSH-Sepharose, Cleavage of GST-SUMO and Gel filtration
Calculated molecular mass:
Mono-Isotopic Mass: 24,981.69 daltons [After tag cleavage]
Average Mass: 24, 997.29 daltons [After tag cleavage]
Purity:
>80 %
Storage Buffer:
50 mM HEPES pH 8, 150 mM NaCL,10 µM GDP, 0. 5m M TCEP
Storage Temperature:
-70 °C
Theoretical PI:
6.08 [After tag cleavage]
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – C225 (end) of human RAB32. Residue M325 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. SUMO is located at residues 229 - 324.
Protease Cleavage:
SENP1 cleavage of SUMO
Cloning Sites:
BamH1 and Xho1 sites of pGex6P-1-SUMO

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