GSH-Sepharose, Cleavage of GST-SUMO and Gel filtration
Calculated molecular mass:
Mono-Isotopic Mass: 24,981.69 daltons [After tag cleavage]
Average Mass: 24, 997.29 daltons [After tag cleavage]
50 mM HEPES pH 8, 150 mM NaCL,10 µM GDP, 0. 5m M TCEP
6.08 [After tag cleavage]
Gel Information :
Gel Image 1:
Amino acids M1 – C225 (end) of human RAB32.
Residue M325 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
SUMO is located at residues 229 - 324.
SENP1 cleavage of SUMO
BamH1 and Xho1 sites of pGex6P-1-SUMO
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