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Recombinant Protein - ROCK I (1 - 535)
Name: ROCK I (1 - 535)
Price per aliquot (100µg): £110.00
Rho associated coiled-coli containing protein kinase 1
ROCK1, p160ROCK, ROCKI
Catalog Number (DU Number):
Tag and Terminus:
Enzymatic Assay Format:
32P filter binding
Long S6 peptide (KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK)
Calculated molecular mass:
Mono-Isotopic Mass: 89, 981.87 daltons
Average Mass: 90, 039.56 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
-80 deg C
Amino acids M1 – L535 (end residue S1354) of human ROCK1.
Residue M243 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
PreScission site (LEVLFQGP) residues 221 – 228
Not1 sites of pFastBac GST 6P2
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