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Recombinant Protein - ROCK I (1 - 535)

Name: ROCK I (1 - 535)
Price per aliquot (100µg): £110.00
Long Name:
Rho associated coiled-coli containing protein kinase 1
Synonyms:
ROCK1, p160ROCK, ROCKI
Catalog Number (DU Number):
DU51657
Accession:
NM_005406.2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Enzymatic Assay Format:
32P filter binding
Enzymatic Substrate:
Long S6 peptide (KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK)
Calculated molecular mass:
Mono-Isotopic Mass: 89, 981.87 daltons
Average Mass: 90, 039.56 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.29
Native Sequence:
Amino acids M1 – L535 (end residue S1354) of human ROCK1. Residue M243 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission site (LEVLFQGP) residues 221 – 228
Cloning Sites:
Not1 sites of pFastBac GST 6P2

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