Recombinant Protein - CLK1 (129 - 484)

Name: CLK1 (129 - 484)
Price per aliquot (100µg): £100.00
Synonyms:
CDC like kinase 1
Catalog Number (DU Number):
DU27352
Accession:
NM_004071.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
RNRYRDVSPFDHSR Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 68, 555.83 daltons
Average Mass: 68, 600.03 daltons
Protein Activity:
Constitutively Active
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical pI:
6.57
Native Sequence:
Amino acids H129 – I484 (end) of human CLK1. Residue H233 of the fusion protein is equivalent to H129 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGex6P-1

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