Recombinant Protein - SRPK3 (1 - 567)

Name: SRPK3 (1 - 567)
Price per aliquot (100µg): £110.00
Long Name:
SRSF protein kinase 3
Synonyms:
serine/threonine kinase 23, SFRS protein kinase 3, STK23, MSSK1
Catalog Number (DU Number):
DU27340
Accession:
NM_014370.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Substrate:
RSRSRSRSRSRSRSR residues 204 – 218 of human ASF-1/SF-2
Calculated molecular mass:
Mono-Isotopic Mass: 88, 781.82 daltons
Average Mass: 88, 838.35 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
6.43
Native Sequence:
Amino acids M1 – P567 (end) of human SRPK3. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGex6P1

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