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Recombinant Protein - EPH A2 (591 - 976) D757A

Name: EPH A2 (591 - 976) D757A
Price per aliquot (100µg): £100.00
Long Name:
EPH receptor A2
Synonyms:
EPHA2
Catalog Number (DU Number):
DU53693
Accession:
NM_004431
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 0.1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 70, 310.93 daltons
Average Mass: 70, 356.52 daltons
Protein Activity:
Kinase/Ligase/DUB Dead
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical pI:
7.21
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids P591 – I976 (end) of human EPH A2. Residue P232 of the fusion protein is equivalent to P591 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a D757A mutation, which produce a kinase dead enzyme. Residue D757 is equivalent to A398 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGPL) residues 221 - 229
Cloning Sites:
BglIII and Sal1site of pGEX 6P-1

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