Recombinant Protein - PRKG2 (1 - 762)

Name: PRKG2 (1 - 762)
Price per aliquot (100µg): £110.00
Long Name:
protein kinase, cGMP-dependent, type II
Synonyms:
cGKII, PRKGR2
Catalog Number (DU Number):
DU26294
Accession:
Q13237.1
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Substrate:
KKRKSSLRRWSPLTPRQMSFDC
Calculated molecular mass:
Mono-Isotopic Mass: 90, 746.61 daltons
Average Mass: 90, 803.79 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
8.43
Native Sequence:
Amino acids M1 – F762 (end) of human PRKG2. Residue M29 of the fusion protein is equivalent to M1 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
BglII and Not1 into BamH1 and Not1 sites of pFastBac HTb

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