Recombinant Protein - MET (956 - 1390)

Name: MET (956 - 1390)
Price per aliquot (100µg): £110.00
Long Name:
MET proto-oncogene, receptor tyrosine kinase
Synonyms:
DFNB97, hepatocyte growth factor receptor, HGFR, RCCP2
Catalog Number (DU Number):
DU35382
Accession:
NM_000245.3
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
GGMEDIYFEFMGGKKK Final concentration: 300 uM
Calculated molecular mass:
Mono-Isotopic Mass: 75, 805.45 daltons
Average Mass: 75, 854.21 daltons
Protein Activity:
Constitutively Active
Purity:
6.21
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
6.50
Native Sequence:
Amino acids K956 – S1390 (end) of human MET. Residue K232 of the fusion protein is equivalent to K956 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission site (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pFastBac GST 6P1

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