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Recombinant Protein - PRKG1 isoform 2 (1 - 686)

Name: PRKG1 isoform 2 (1 - 686)
Price per aliquot (100µg): £110.00
Long Name:
cGMP-dependent, type 1
Synonyms:
PRKG1B, PRKGR1B, PGK, PKG
Catalog Number (DU Number):
DU 26285
Accession:
NM_006258.3
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Substrate:
KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK
Calculated molecular mass:
Mono-Isotopic Mass: 81, 124.05 daltons
Average Mass: 81, 175.47 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical PI:
5.37
Native Sequence:
Amino acids M1 – F686 (end) of human PRKG1 isoform 2. Residue M29 of the fusion protein is equivalent to M1 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
BamH1 and Not1 sites of pFastBac HTb

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