Recombinant Protein - RAB43 (1 - 212)

Name: RAB43 (1 - 212)
Price per aliquot (100µg): £110.00
Long Name:
RAB43, member RAS oncogene family
Synonyms:
ISY1, RAB11B, RAB41
Catalog Number (DU Number):
DU26431
Accession:
NM_198490.2
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved

N-Term
SUMO
Uncleaved
Purification Method:
Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 35, 398.64 daltons
Average Mass: 35, 420.86 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical PI:
5.74
Native Sequence:
Amino acids M1 – C212 (end) of human RAB43. Residue M105 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 2 – 7.
Protease Cleavage:
SENP1 cleavage of SUMO
Cloning Sites:
BamH1 and Not1 site of pET15-His-SUMO

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