COVID-19 CLOSURE

COVID-19 Closure

Due to the current situation with Covid 19 this facility is currently closed and all staff are working from home. Although we will be checking emails regularly, there may be a longer delay than usual in replying.

Recombinant Protein - RAB43 (1 - 212)

Name: RAB43 (1 - 212)
Price per aliquot (100µg): £110.00
Long Name:
RAB43, member RAS oncogene family
Synonyms:
ISY1, RAB11B, RAB41
Catalog Number (DU Number):
DU26431
Accession:
NM_198490.2
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved

N-Term
SUMO
Uncleaved
Purification Method:
Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 35, 398.64 daltons
Average Mass: 35, 420.86 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-80 deg C
Theoretical PI:
5.74
Native Sequence:
Amino acids M1 – C212 (end) of human RAB43. Residue M105 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 2 – 7.
Protease Cleavage:
SENP1 cleavage of SUMO
Cloning Sites:
BamH1 and Not1 site of pET15-His-SUMO

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