Recombinant Protein - IRAK4 (1 - 460) D329A

Name: IRAK4 (1 - 460) D329A
Price per aliquot (100µg): £110.00
Long Name:
Interleukin-1 Receptor-Associated Kinase 4 (IRAK4)
Catalog Number (DU Number):
DU8886
Accession:
AAH13316
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM magnesium acetate
Enzymatic Substrate:
MBP Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 78, 259.58 daltons
Average Mass: 78, 309.89 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.44
Native Sequence:
Amino acids M1 – S460 (end) of human IRAK4. Residue V232 of the fusion protein is equivalent to V160 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a D329A mutation, which produce a kinase dead enzyme. Residue D168A is equivalent to A560 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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