Recombinant Protein - JNK1 K55R K56R (1 - 384)

Name: JNK1 K55R K56R (1 - 384)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU47326
Accession:
L26318
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 71, 063.24 daltons
Average Mass: 71, 109.43 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
6.49
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – Q384 (end) of human JNK1. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a K55R and a K56R mutation. Residue K55 is equivalent to R286 and K56 is equivalent to R287 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 – 228
Cloning Sites:
BamH1 and Not1 sites of pGEX6P-1

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