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Recombinant Protein - Calmodulin 2 (1 - 149)

Name: Calmodulin 2 (1 - 149)
Price per aliquot (100µg): £110.00
Synonyms:
CALM2, PHKD, phosphorylase kinase delta
Catalog Number (DU Number):
DU45677
Accession:
NM_001743
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Calculated molecular mass:
Mono-Isotopic Mass: 19,619.02
Average Mass: 19,631.58
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical PI:
4.39
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – K149 (end) of human. Residue M26 of the fusion protein is equivalent to M1 of the native enzyme. The His6 tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 14 - 20
Cloning Sites:
BamH1 and Not1 sites of pET15b

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