Recombinant Protein - ERK5 (1 - 816)

Name: ERK5 (1 - 816)
Price per aliquot (100µg): £110.00
Synonyms:
MAPK7
Catalog Number (DU Number):
DU 842
Accession:
NM_002749
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Myelin Basic Protein (MBP) Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 89, 284.48 daltons
Average Mass: 89, 340.51 daltons
Protein Activity:
Has been Activated during expression
Purity:
80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical pI:
5.81
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – P816 (end) of human ERK5. Residue M8 of the fusion protein is equivalent to M1 of the native enzyme. The His6 tag is located at residues 2 - 7.
Protease Cleavage:
None
Cloning Sites:
BamH1 and Sal1 sites of pFastBac1

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