Recombinant Protein - HIPK4 (1 - 616)

Name: HIPK4 (1 - 616)
Price per aliquot (100µg): £110.00
Synonyms:
HIPK4
Catalog Number (DU Number):
DU5222
Accession:
NM_144685
Expression:
bacteria
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
Myelin basic protein Final concentration: 0.3 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 73, 094.50 daltons
Average Mass: 73, 141.00 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical PI:
6.06
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – H616 (end) of human HIPK4. Residue M31 of fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
EcoR1 and Not1 site of pFastBAC HTa

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