FESTIVE CLOSURE

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Recombinant Protein - DYRK1b (1 - 589)

Name: DYRK1b (1 - 589)
Price per aliquot (100µg): £100.00
Synonyms:
DYRK1B
Catalog Number (DU Number):
DU12362
Accession:
NM_006483.1
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
Glutathione Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc,
Enzymatic Substrate:
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 91, 699.89 daltons
Average Mass: 91, 758.36 daltons
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
8.66
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S589 (end) of human DYRK1b. Residue M232 of fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and EcoR1 site of pFastBAC GST

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