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Recombinant Protein - 4E-BP1 (1 - 118)

Name: 4E-BP1 (1 - 118)
Price per aliquot (100µg): £110.00
Long Name:
Eukaryotic Translation Initiation Factor 4E binding protein 1 [1 – 118]
Synonyms:
EIF4EBP1
Catalog Number (DU Number):
DU10998
Accession:
NM_004095.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for mTOR
Calculated molecular mass:
Mono-Isotopic Mass: 39,936.02
Average Mass: 39,961.81
Protein Activity:
Constitutively Active
Purity:
>80%
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.51
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – I118 (end) of human 4E-BP1. Residue M237 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and Not1sites of pGEX 6P-1

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