Recombinant Protein - CK1 gamma 2 (1 - 415)

Name: CK1 gamma 2 (1 - 415)
Price per aliquot (100µg): £100.00
Synonyms:
CSNK1G2
Catalog Number (DU Number):
DU35306
Accession:
BC0186932
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
N-terminal GST
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
KRRRALS*VASLPGL (where S* is phospho Ser) Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 74, 233.82 daltons
Average Mass: 74, 281.43 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
8.56
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – K415 of human CSNK1G2. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and Not1 sites of pGEX 6P-1

Do you need any help? Please get in touch and we’ll be happy to lend a hand.