FESTIVE CLOSURE

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Recombinant Protein - CK1 alpha 1 (2 - 337)

Name: CK1 alpha 1 (2 - 337)
Price per aliquot (100µg): £100.00
Synonyms:
CSNK1A1
Catalog Number (DU Number):
DU329
Accession:
X80693
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KRRRALS*VASLPGL (where S* is phospho Ser) Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 65, 565.48 daltons
Average Mass: 65, 607.83 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
9.02
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids A2 – F337 (end) of human CK1 alpha 1. Residue A232 of the fusion protein is equivalent to A2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 - 228
Cloning Sites:
BamH1 and Not1 sites of pGEX-6P-1

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