FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - CaMK4 (1 - 473)

Name: CaMK4 (1 - 473)
Price per aliquot (100µg): £100.00
Long Name:
calcium / calmodulin-dependent protein kinase IV
Synonyms:
CAMK4
Catalog Number (DU Number):
DU 35125
Accession:
NM_001744.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM CaCl2, 1 μM Calmodulin, 10 mM MgAc,
Enzymatic Substrate:
KKLNRTLSVA; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 79,677.99
Average Mass: 79,728.80
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.71
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – Y473 (end) of human CAMK4. Residue M243 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 - 228
Cloning Sites:
Not1 sites of pGEX-6P-2

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