FESTIVE CLOSURE

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Recombinant Protein - CaMK2D (1 - 478)

Name: CaMK2D (1 - 478)
Price per aliquot (100µg): £100.00
Synonyms:
CAMK2D
Catalog Number (DU Number):
DU33795
Accession:
NM_172115.2
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc, 0.1 mM CaCl2, 1 μM Calmodulin
Enzymatic Substrate:
YLRRRLSDSNF; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 80928.15
Average Mass: 80980.12
Protein Activity:
Constitutively Active
Purity:
85%
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
6.41
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – K478 (end) of human CaMK2D. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. The following amino acid substitution is present: A – V, where A151 of native enzyme is V382 of the fusion protein
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
BamH1 and Not1 sites of pGEX 6P-1

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