50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc, 0.1 mM CaCl2, 1 μM Calmodulin
YLRRRLSDSNF; Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 80928.15
Average Mass: 80980.12
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Gel Information :
Gel Image 1:
Amino acids M1 – K478 (end) of human CaMK2D. Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. The following amino acid substitution is present:
A – V, where A151 of native enzyme is V382 of the fusion protein
PreScission (LEVLFQGP) residues 221 - 229
BamH1 and Not1 sites of pGEX 6P-1
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