FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - BRK (1 - 451)

Name: BRK (1 - 451)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU5916
Accession:
NM_005975
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc, 5 mM MnCl2
Enzymatic Substrate:
Poly Glu:Tyr (4:1); Final concentration: 1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 55,170.60
Average Mass: 55,205.80
Protein Activity:
Constitutively Active
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 10 mM DTT, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
-70 °C
Theoretical pI:
6.41
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – T451 (end) of human BRK. Residue M29 of fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 - 24
Cloning Sites:
BamH1 and Not1 site of pFastBAC HTb

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