Recombinant Protein - EPH A4 (570 - 986)

Name: EPH A4 (570 - 986)
Price per aliquot (100µg): £110.00
Long Name:
EPH receptor A4
Synonyms:
Hek8, TYRO1
Catalog Number (DU Number):
DU4580
Accession:
NM_004438
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 mM EGTA, 0.1 % 2−mercaptoethanol, 10 mM MgAc
Enzymatic Substrate:
Poly Glu Tyr (4:1) Final concentration: 0.1 mg/ml
Calculated molecular mass:
Mono-Isotopic Mass: 74, 567.72 daltons
Average Mass: 74, 615.92 daltons
Protein Activity:
Constitutively Active
Purity:
90 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 °C
Theoretical pI:
6.73
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids S570 – V986 (end) of human EPH A4. Residue S238 of the fusion protein is equivalent to S570 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 229
Cloning Sites:
Sal1 and Not1site of pGEX 6P-3

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