50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
KKLNRTLSFAEPG Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 68, 784.45 daltons
Average Mass: 68, 828.22 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Gel Information :
Gel Image 1:
Amino acids M1 – L363 of human DAPK1
[Full length protein ends at residue R1430]
Residue M235 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
PreScission (LEVLFQGP) residues 221 - 228
EcoRI and NotI sites of pGEX-6P-1
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