FESTIVE CLOSURE

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Recombinant Protein - DAPK1 (1 - 363)

Name: DAPK1 (1 - 363)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU31113
Accession:
NM_0049382
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KKLNRTLSFAEPG Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 68, 784.45 daltons
Average Mass: 68, 828.22 daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical pI:
5.96
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – L363 of human DAPK1 [Full length protein ends at residue R1430] Residue M235 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
EcoRI and NotI sites of pGEX-6P-1

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