FESTIVE CLOSURE

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Recombinant Protein - CK1 delta (1 - 415)

Name: CK1 delta (1 - 415)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU19064
Accession:
NM_001893
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KRRRALS*VASLPGL (where S* is phospho Serine) Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 74, 106.86 daltons
Average Mass: 74, 154.24daltons
Protein Activity:
Constitutively Active
Purity:
85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
–70 °C
Theoretical pI:
9.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – R415 of human CK1 delta. Residue M232 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamHI and NotI sites of pGEX-6PB-1

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