50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
ABLtide [EAIYAAPFAKKK], Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 74,424.49
Average Mass: 74,472.32
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Gel Information :
Gel Image 1:
Amino acids P118 – S535 of human ABL1. [Full length protein ends at residue R1130].
Residue P232 of the fusion protein is equivalent to P2 of the native enzyme. The GST tag is located at residues 1 – 220.
Prescission site (LEVLFQGP) at residues 221 - 228
BamH1 and EcoR1 sites of pGEX-6P-1
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