FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - ABL (118 - 535)

Name: ABL (118 - 535)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU5578
Accession:
NM_005157
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
ABLtide [EAIYAAPFAKKK], Final concentration: 300 μM
Calculated molecular mass:
Mono-Isotopic Mass: 74,424.49
Average Mass: 74,472.32
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
6.00
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids P118 – S535 of human ABL1. [Full length protein ends at residue R1130]. Residue P232 of the fusion protein is equivalent to P2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 - 228
Cloning Sites:
BamH1 and EcoR1 sites of pGEX-6P-1

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