FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - DGK zeta (1 - 929)

Name: DGK zeta (1 - 929)
Price per aliquot (100µg): £100.00
Synonyms:
DGKZ
Catalog Number (DU Number):
DU19618
Accession:
BC0417701
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni 2+ -NTA agarose
Enzymatic Assay Format:
ADP Glo
Enzymatic Buffer:
20 mM Tris-HCl, 67 mM KCl, 1 mM DTT, 0.05 mg/ml BSA, 10 mM MgCl2
Enzymatic Substrate:
Diacylglycerol Final concentration: 0.1 mM
Calculated molecular mass:
Mono-Isotopic Mass: 107, 412.86 daltons
Average Mass: 107, 480.73 daltons
Purity:
>80 %
Storage Buffer:
50 mM HEPES pH 7.0, 150 mM NaCl, 20 % glycerol, 5 mM DTT
Storage Temperature:
-70 °C
Theoretical pI:
8.16
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – V929 (end) of human DGK zeta. Residue M29 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
Protease Cleavage:
rTEV (ENLYFQG) residues 18 – 24
Cloning Sites:
BgII1 - Not1 to BamH1 - Not1 sites in pFastBAC HTb

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