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Recombinant Protein - DGK gamma (1 -752)
Name: DGK gamma (1 -752)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
Ni -NTA agarose
Enzymatic Assay Format:
20 mM Tris-HCl, 67 mM KCl, 1 mM DTT, 0.05 mg/ml BSA, 10 mM MgCl
Diacylglycerol Final concentration: 0.1 mM
Calculated molecular mass:
Mono-Isotopic Mass: 88, 804.77 daltons
Average Mass: 88, 862.29 daltons
50 mM HEPES pH 7.0, 150 mM NaCl, 20 % glycerol, 5 mM DTT
Amino acids M1 – D752 (end) of human DGK gamma.
Residue M26 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10.
rTEV (ENLYFQG) residues 18 – 24
Sal1 and Not1 sites in pFastBAC HTc
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