50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 84, 458.95 daltons
Average Mass: 84, 513.34 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Gel Information :
Gel Image 1:
Amino acids M1 – S502 of human DYRK1a.
[Full length protein ends at residue V7540]
Residue M232 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220 of the fusion protein.
PreScission (LEVLFQGP) residues 221 - 228
BamH1 and Not1site of pGEX6P-1
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