FESTIVE CLOSURE

Festive Closure

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Recombinant Protein - DYRK1a (1 - 502)

Name: DYRK1a (1 - 502)
Price per aliquot (100µg): £100.00
Catalog Number (DU Number):
DU19040
Accession:
NM_1304372
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
WOODtide [KKISGRLSPIMTEQ] Final concentration: 500 μM
Calculated molecular mass:
Mono-Isotopic Mass: 84, 458.95 daltons
Average Mass: 84, 513.34 daltons
Protein Activity:
Constitutively Active
Purity:
>70 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-70 °C
Theoretical pI:
8.68
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S502 of human DYRK1a. [Full length protein ends at residue V7540] Residue M232 of the fusion protein is equilivalent to M1 of the native enzyme. The GST tag is located at residues 1 - 220 of the fusion protein.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and Not1site of pGEX6P-1

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