Recombinant Protein - MO25 alpha (1 - 341) R227A

Name: MO25 alpha (1 - 341) R227A
Price per aliquot (100µg): £110.00
Synonyms:
CAB39
Catalog Number (DU Number):
DU17691
Accession:
NM_016289.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 66, 565.31 daltons
Average Mass: 66, 608.05 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-80 deg C
Theoretical pI:
6.00
Native Sequence:
Amino acids M1 – A341 (end) of human MO25 alpha. Residue M232 of fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a R227A mutation. Residue R227 is equivalent to A458 of the fusion protein.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 – 228
Cloning Sites:
BamH1 sites of pGex6P-1

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