Recombinant Protein - Inhibitor 2 (1 - 205)

Name: Inhibitor 2 (1 - 205)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU3247
Accession:
NP_006232
Expression:
bacteria
Purification Method:
Boil (15 mins), Q-Sepharose and Mono-Q Method based on: Helps, NR. Street, AJ. Elledge, SJ. And Cohen, PTW. (1994). “Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli” FEBS letters. 340. 93 - 98.
Enzymatic Assay Format:
Standard phosphatase assay IC50 of 1 nM against PP1 gamma using [ 32P] phosphorylase a as substrate
Calculated molecular mass:
Mono-Isotopic Mass: 23, 001.8 daltons
Average Mass: 23, 016.13 daltons
Protein Activity:
Constitutively Active
Publication info:
Publication Name: 
@Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli”
PMID: 
8119416
Purity:
90 %
Storage Buffer:
20 mM Ammonium acetate pH 7, 0.1 mM EGTA, 1 mM DTT
Storage Temperature:
-70 °C
Theoretical PI:
4.57
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – S205 (end) of human Inhibitor 2.
Protease Cleavage:
None
Cloning Sites:
Nde1 and BglII of pCW

Do you need any help? Please get in touch and we’ll be happy to lend a hand.