Recombinant Protein - IkappaB alpha (2 - 54)

Name: IkappaB alpha (2 - 54)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1442
Accession:
BC004983
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 33, 150.71 daltons
Average Mass: 33, 172.22 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical pI:
5.24
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids F2 – L54 (end residue is L317) of human IkappaB alpha. Residue F232 of the fusion protein is equivalent to F2 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamH1 and EcoR1 sites of pGEX6P-1

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