Recombinant Protein - IkappaB alpha

Name: IkappaB alpha
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU1007
Accession:
BC004983
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Substrate for IKK beta
Calculated molecular mass:
Mono-Isotopic Mass: 62, 262.10 daltons
Average Mass: 62, 301.90 daltons
Purity:
> 85 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine.
Storage Temperature:
–70 °C
Theoretical PI:
4.88
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids F2 – L317 of human IkappaB alpha. Residue F232 of the fusion protein is equivalent to M1 of the native protein. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
BamHI and EcoR1 sites of pGEX-6P-1

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